Journal: Biochemistry and Biophysics Reports

Article Title: 2- O -α-D-Glucopyranosyl- l -ascorbic acid as an antitumor agent for infusion therapy

doi: 10.1016/j.bbrep.2017.04.014

Figure Lengend Snippet: Tumor growth in colon-26 tumor-bearing CDF1 mice treated with AA or AA-2G (equimolecular amount of 300 mg/kg of AA). Tumor sizes (A) and body weight (B) were measured for 7 days during treatment. All data represent means ± SE (n =16). (* P <0.05; ** P <0.01, compared with the control).

Article Snippet: When transplanted tumor sizes were more than 10 mm in diameter, the tumors were excised and small pieces of the tumor (approximately 2-mm cubes) were engrafted subcutaneously into the left dorsal area of each five-week-old male CDF1 mouse (Japan SLC, Shizuoka, Japan).

Techniques: Control

Journal: Biochemistry and Biophysics Reports

Article Title: 2- O -α-D-Glucopyranosyl- l -ascorbic acid as an antitumor agent for infusion therapy

doi: 10.1016/j.bbrep.2017.04.014

Figure Lengend Snippet: In vivo biodistribution and clearance of AA-2G in colon-26 tumor-bearing CDF1 mice. AA-2G was injected intravenously into colon-26 tumor-bearing CDF1 mice. AA levels in the liver, kidney and tumor (A), AA level in plasma (B), AA-2G levels in tissues and plasma (C), and blood glucose level (D) after administration of AA-2G. All data represent means ± SE (n =4). A significant difference was found between kidney AA levels at 15 min and 0 min (A; P <0.05). Other significant differences are indicated in the figure (* P <0.05, each time point compared with that at 0 min).

Article Snippet: When transplanted tumor sizes were more than 10 mm in diameter, the tumors were excised and small pieces of the tumor (approximately 2-mm cubes) were engrafted subcutaneously into the left dorsal area of each five-week-old male CDF1 mouse (Japan SLC, Shizuoka, Japan).

Techniques: In Vivo, Injection, Clinical Proteomics

Journal: Biochemistry and Biophysics Reports

Article Title: 2- O -α-D-Glucopyranosyl- l -ascorbic acid as an antitumor agent for infusion therapy

doi: 10.1016/j.bbrep.2017.04.014

Figure Lengend Snippet: Time course of plasma MDA level in colon-26 tumor-bearing CDF1 mice after injection of AA-2G. MDA levels in plasma of tumor-bearing mice after injection of AA-2G and AA were determined. All data represent mean concentration ± SE (n =4).

Article Snippet: When transplanted tumor sizes were more than 10 mm in diameter, the tumors were excised and small pieces of the tumor (approximately 2-mm cubes) were engrafted subcutaneously into the left dorsal area of each five-week-old male CDF1 mouse (Japan SLC, Shizuoka, Japan).

Techniques: Clinical Proteomics, Injection, Concentration Assay

Journal: Stem cells (Dayton, Ohio)

Article Title: Hyaluronic acid facilitates the recovery of hematopoiesis following 5-fluorouracil administration.

doi: 10.1634/stemcells.22-4-544

Figure Lengend Snippet: Figure 1. Hyaluronic acid (HA) facilitates recovery of peripheral blood cells after 5-fluorouracil (5-FU) administration. B6D2F1 hybrid mice (n = 10 per group) were administered 5-FU (150 mg/kg) on day 0. HA (100 µg/mouse) or phosphate buffered-saline (PBS) was injected on day 4, 6, 10, and 13. Blood was harvested daily, and the numbers of (A) leukocytes (white blood cells [WBCs]) and (C) thrombocytes (platelets [PLTs]) were measured and expressed as mean ± standard deviation (SD). Control measurements from untreated mice were taken on day 0, before 5-FU administration. To demonstrate a dose-dependent effect of HA on WBC recovery, mice (n = 5 per group) were administered with various doses of HA (0–1,000 µg/mouse), and the peripheral blood samples were evalu- ated for the leukocyte number on day 7; cell counts are expressed as mean ± SD (B). A significant difference (*p < .01) in cell counts between 5FU/PBS and 5FU/HA groups was detected for WBCs from day 6 to day 12 and for PLTs from day 6 to day 11. The results of one representative out of three similar experiments are shown.

Article Snippet: Eightto 12week-old female mice C57Bl/6J ✕ DBA/2J (B6D2F1) were obtained from Harlan Inc. (Indianapolis, IN) or bred inhouse.

Techniques: Saline, Injection, Standard Deviation, Control

Journal: Stem cells (Dayton, Ohio)

Article Title: Hyaluronic acid facilitates the recovery of hematopoiesis following 5-fluorouracil administration.

doi: 10.1634/stemcells.22-4-544

Figure Lengend Snippet: Figure 2. Hyaluronic acid (HA) facilitates bone marrow recovery after 5-fluorouracil (5-FU) treatment. B6D2F1 hybrid mice (a total number of 40) were administered 5-FU (150 mg/kg) on day 0. HA (100 µg/mouse) or phosphate-buffered saline (PBS) was injected on day 4, 6, 10, and 13. (A):The bone marrow of control, untreated mice (n = 5) was analyzed on day 0. On day 7, 14, 21, and 28, bone marrow cells (Bmcs) were harvested from 5FU/PBS and 5FU/HA mice (n = 5 for every group per each time point), counted, and expressed as mean ± standard deviation (SD). The results of one representa- tive out of two similar experiments are shown. (B): Bmcs from 5FU/PBS and 5FU/HA mice were harvested on day 7, assayed for numbers of granulocyte-macrophage colony-forming unit (CFU-GM) and BFU-E in methylcellulose media, and presented as mean ± SD. (C): Smears of bone marrow harvested at day 7 were fixed and stained with Giemsa dye. Numbers of blast cells and megakaryocytes (MKC) were calculated and expressed as the percentage of cells examined. (D): Bmcs were harvested from 5FU/PBS and 5FU/HA mice (n = 3) on day 7 and assayed for number of long-term culture- initiating cells (LTC-ICs) in the limiting dilution assay in two independent experiments. The LTC-IC frequency in the bone marrow from one representative experiment is shown as mean ± SD. A statistically significant difference is indicated by an asterisk.

Article Snippet: Eightto 12week-old female mice C57Bl/6J ✕ DBA/2J (B6D2F1) were obtained from Harlan Inc. (Indianapolis, IN) or bred inhouse.

Techniques: Saline, Injection, Control, Standard Deviation, Staining, Limiting Dilution Assay

Journal: Stem cells (Dayton, Ohio)

Article Title: Hyaluronic acid facilitates the recovery of hematopoiesis following 5-fluorouracil administration.

doi: 10.1634/stemcells.22-4-544

Figure Lengend Snippet: Figure 3. 5-Fluorouracil (5-FU) interferes with the cell surface expression of CD44. B6D2F1 mice were administered 150 mg/kg 5-FU (day 0). Bone marrow cells were harvested on days 2 and 4. (A): The expression of pan-CD44 was detected by a CD44-specific anti- body (transparent field) and analyzed by flow cytometry. Isotype-matched immunoglobulin G was used as a negative control (black field). Histograms represent a fluorescent intensity of total nongated bone marrow cells from control, nontreated mice and 5-FU–treated mice (days 2 and 4). (B): The expression of CD44v6 on gated R4 subpopulation of bone marrow cells from control and 5-FU–-treated (day 2) mice is shown.

Article Snippet: Eightto 12week-old female mice C57Bl/6J ✕ DBA/2J (B6D2F1) were obtained from Harlan Inc. (Indianapolis, IN) or bred inhouse.

Techniques: Expressing, Flow Cytometry, Negative Control, Control

Journal: Stem cells (Dayton, Ohio)

Article Title: Hyaluronic acid facilitates the recovery of hematopoiesis following 5-fluorouracil administration.

doi: 10.1634/stemcells.22-4-544

Figure Lengend Snippet: Figure 4. Hyaluronic acid (HA) binds bone marrow cells in vivo. B6D2F1 mice were administered 150 mg/kg 5-fluorouracil. (A): On day 4, HA labeled with fluorescein isothiocyanate (HA-FITC) was injected intravenously; 2 hours later bone marrow and peripheral blood cells were harvested, and the cell samples were examined using flow cytometry (transparent field). The fluorescent activity of hyaluronidase-treated cells is shown as a dark field. The fluorescent signal was analyzed with CellQuest- Pro (Becton, Dickinson). One representative histogram out of three is shown. (B): HA-FITC (fluorescein isothiocyanate) bind- ing by CD11b (right) and CD31 (left) positive cells (FL2) was evaluated by fluorescence-activated cell sorter analysis. One rep- resentative dot plot out of three is shown. (C): Expression of CD44s, CD44v4, CD44v6, and CD44v10 (FL1) on bone marrow-derived macrophages is shown.

Article Snippet: Eightto 12week-old female mice C57Bl/6J ✕ DBA/2J (B6D2F1) were obtained from Harlan Inc. (Indianapolis, IN) or bred inhouse.

Techniques: In Vivo, Labeling, Injection, Flow Cytometry, Activity Assay, Fluorescence, Expressing, Derivative Assay

Journal: Stem cells (Dayton, Ohio)

Article Title: Hyaluronic acid facilitates the recovery of hematopoiesis following 5-fluorouracil administration.

doi: 10.1634/stemcells.22-4-544

Figure Lengend Snippet: Figure 5. Hyaluronic acid (HA) upregulates expression of interleukin-1 (IL-1) and IL-6 in the bone marrow. B6D2F1 mice were administered 150 mg/kg 5-fluorouracil (5-FU). On day 4, the mice were administered 100 µg/mouse HA, chondroitin sulfate (CS), or 200 µ1 phosphate-buffered saline (PBS). Bone marrow cells were harvested, and total RNA was isolated. (A): The number of IL-1α, IL-6, and β-actin transcripts were analyzed by northern blot. (B): Quantification of northern hybridization signals was done with Scion Image PC (“Image 2”) software (version1.6.1, NIH, Bethedsa, MD). (C, D): Serum levels of IL-1 and IL-6 were examined by enzyme-linked immunosorbent assay and expressed as mean ± standard deviation (SD). (E): The colony-promoting activity of serum samples was examined in colony-forming unit (CFU) assay in the presence of 5% WEHI- 3B condition media. Where indicated, IL-1- and IL-6-specific neutralizing antibodies (100 µg/ml) were added. The number of colonies is expressed as mean ±SD. A statistically significant difference is indicated by an asterisk.

Article Snippet: Eightto 12week-old female mice C57Bl/6J ✕ DBA/2J (B6D2F1) were obtained from Harlan Inc. (Indianapolis, IN) or bred inhouse.

Techniques: Expressing, Saline, Isolation, Northern Blot, Hybridization, Software, Enzyme-linked Immunosorbent Assay, Standard Deviation, Activity Assay, Colony-forming Unit Assay

Journal: Cancer Science

Article Title: Enhanced antitumor efficacy of folate‐linked liposomal doxorubicin with TGF‐β type I receptor inhibitor

doi: 10.1111/j.1349-7006.2010.01646.x

Figure Lengend Snippet: Biodistribution of doxorubicin (DXR) at 24 h after intravenous injection of liposomal DXR (5 mg DXR/kg) with or without intraperitoneal injection of A‐83‐01 (0.5 mg A‐83‐01/kg) into CDF1 mice bearing M109 tumors. Each value represents the mean ± SD. (n = 3). **P < 0.01, *P < 0.05.

Article Snippet: M109 cells (1.5 × 10 6 ) were inoculated subcutaneously into CDF1 female mice (5 weeks old, Sankyo Labo Service, Tokyo, Japan).

Techniques: Injection

Journal: Cancer Science

Article Title: Enhanced antitumor efficacy of folate‐linked liposomal doxorubicin with TGF‐β type I receptor inhibitor

doi: 10.1111/j.1349-7006.2010.01646.x

Figure Lengend Snippet: Effects of A‐83‐01 on the antitumor activity of a single injection of liposomal doxorubicin (DXR) in CDF1 mice bearing M109 tumors. Free DXR, liposomal DXR (8 mg/kg), or saline was administered intravenously in a single bolus with or without intraperitoneal A‐83‐01 injection (0.5 mg/kg) to mice (n = 6–8). (a) Tumor size; (b) enlargement of (a). (c) Body weight change after drug administration. The formulations used were saline (⋄), saline/A‐83‐01 (♦), free DXR (□), non‐folate‐linked PEGylated liposomal doxorubicin (SL) (△), SL/A‐83‐01 (), folate‐linked liposomal doxorubicin (F‐SL) (○), F‐SL/A‐83‐01 (•). #P < 0.05, ##P < 0.01 versus SL, †P < 0.05 versus SL/A‐83‐01, *P < 0.05, **P < 0.01 versus F‐SL. Each value represents the mean ± SD.

Article Snippet: M109 cells (1.5 × 10 6 ) were inoculated subcutaneously into CDF1 female mice (5 weeks old, Sankyo Labo Service, Tokyo, Japan).

Techniques: Activity Assay, Injection, Saline